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Forest health is under mounting pressure from diseases driven by invasive pathogens, and the ability to detect these diseases early and precisely is vital for targeted, prompt forest management. Breakthroughs in spectral imaging and artificial intelligence have paved the way for novel approaches to plant disease identification in both crop and tree species. The present study employed the Dutch elm disease (DED; caused by Ophiostoma novo-ulmi) and American elm (Ulmus americana) pathosystem as a model to test the efficacy of two custom-fabricated, high-precision, portable hyper- and multispectral leaf scanners, paired with machine learning, as innovative instruments for forest disease surveillance. Hyper- and multispectral imagery was collected from leaves of American elm genotypes with varying degrees of disease susceptibility following mock inoculation and inoculation with O. novo-ulmi in controlled greenhouse conditions. Both classical machine learning and cutting-edge deep learning architectures were trained on extracted spectra and directly on spectral image volumes. Deep learning frameworks that leverage combined spectral and spatial information from high-definition spectral leaf images surpass classical machine learning approaches that rely exclusively on spectral data for DED identification. Within the deep learning framework, leaf margins and symptomatic lesions were accentuated as discriminative spatial cues for separating leaves originating from inoculated versus mock-inoculated specimens. Furthermore, the spectral and spatial signature profiles uncovered by the machine learning-based models corresponded to the DED susceptibility levels of the elm genotypes. While supplementary investigations are warranted to gauge transferability across other pathosystems, hyper- and multispectral leaf scanners integrated with machine learning show considerable promise as emerging tools for disease phenotyping in tree species.

Global food security is critically threatened by plant diseases, which can be especially catastrophic for smallholder farmers whose economic survival hinges on a productive groundnut crop. It is therefore vital to engineer an algorithm capable of early, automated plant disease diagnosis. Working alongside a pathologist, a large-scale dataset of groundnut leaf imagery was assembled to enable this automated detection. In image classification tasks, Convolutional Neural Networks have demonstrated remarkable efficiency. This investigation constructs a disease identification and classification system using a tri-CNN architecture that incorporates DenseNet169, Inception, and Xception, all of which were initially trained on the ImageNet database. The proposed ensemble strategy formulates final predictions for test data by applying two custom nonlinear functions to the decision outputs of these base learners, integrating their scores across four standard performance measures: recall, precision, accuracy, and F1-score. The tailored CNN models are thus utilized to train our system for superior results. The methodology was tested on a real-world groundnut leaf dataset. The introduced model registered an accuracy reading of 98.46%.